Integrin beta 2 ELISA Kit

Catalog No. ABIN2043773

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Integrin beta 2.

Quick Overview for Integrin beta 2 ELISA Kit (ABIN2043773)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Integrin beta 2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.115 ng/mL

Application Details

Plate: Pre-coated

Protocol: Integrin Beta 2 (ITGb2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 2 (ITGb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 2 (ITGb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 2 (ITGb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Beta 2 (ITGb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation